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Choosing the right vial and closure is important to guarantee trouble-free autosampler operation and reliable, reproducible results in chromatographic analyses.
 

1. What are the most common vial styles for chromatography? 

All DWK Life Sciences chromatography vials are made from clear or amber borosilicate glass, which conforms to USP Type I and ASTM E438, Type I, Class B requirements.
 
Two types of application:
 
HPLC : The most common vial sizes for liquid chromatography vials are 12 x 32 mm as well as 15 x 45 mm. 12 x 32 mm vials will also be described as 1.5 mL, 1.8 mL or 2.0 mL vials.
 
GC: Vials for gas chromatography (headspace vials) are available in different sizes and come with crimp or screw finish. Headspace vials are available with flat and rounded bottom. The rounded bottom is sturdier and therefore more resistant to the high pressure which builds up inside the vial during the heating process.
 

2. How to find the right vial for your autosampler?

 
DWK Life Sciences chromatography vials and accessories are designed for trouble-free operation in most autosamplers. Contact us to get the autosampler compatibility and cross-reference chart and this will help you to find the right vial that suit your equipment.
 

3. Working with light-sensitive samples?

 
DWK Life Sciences line of amber chromatography vials is strongly recommended. Amber glass is formulated to absorb light in the ultraviolet region of the electromagnetic spectrum and therefore provides excellent protection for your valuable samples.
 

4. Working with small-volume samples?

 
The DWK Life Sciences line of chromatography vials offers two different of Limited Volume Insert styles: flat bottom, and conical type if your sample volume should be too small to be analysed using standard chromatography vials.
 

5. Need a writing patch?

 
Writing patches offers an easy option for sample labelling. DWK Life Sciences range of chromatography vials are available with or without writing patch in most of the vial styles.
 

6. What is the difference between Type I and Type III glass?

 
USP Type I classification is a borosilicate glass with superior chemical resistance, also can be used for most applications, including packaging for parenteral and non-parenteral products. Type I glass may be used to package acidic, neutral and alkaline products. Water for injection, unbuffered products, chemicals, sensitive lab samples and those requiring sterilization are commonly packaged in Type I borosilicate glass. Furthermore, Type I glass can be subject to chemical attack under certain conditions as well.
 
While USP Type III is a soda-lime glass with moderate chemical resistance. It is typically acceptable for packaging dry powders that will be dissolved into solutions or buffers that are insensitive to alkali. Type III glass may not be suitable for autoclaved products because the autoclaving process will accelerate the glass corrosion reaction. Dry heat sterilization processes are typically not a problem for Type III containers.